Weight-loss behaviors before pregnancy associate with increased risk of postpartum depression from the Japan Environment and Children’s Study

No studies showed the association between weight-loss behaviors before pregnancy and postpartum depression (PPD). We analyzed data from the nation-wide birth cohort study, the Japan Environment and Children’s Study. Self-administrated questionnaires answered by 62,446 women was analyzed using logistic regression analysis. PPD was assessed using the Edinburgh Postnatal Depression Scale at 1 month after delivery. Women using at least one weight-loss method had higher risk of PPD than women using no weight-loss methods [women without antenatal psychological distress according to Kessler 6-Item Psychological Distress score: adjusted odds ratio (aOR) 1.318, 95% confidence interval (CI) 1.246–1.394; women with antenatal psychological distress: aOR 1.250, 95% CI 0.999–1.565]. Using extremely unhealthy weight-loss methods was associated with PPD compared with no use of each weight-loss method (vomiting after eating: aOR 1.743, 95% CI 1.465–2.065; smoking: aOR 1.432, 95% CI 1.287–1.591; taking diet pills: aOR 1.308, 95% CI 1.122–1.520). The association between weight-loss behaviors and PPD varied according to pre-pregnancy BMI. In normal-weight women, the weight-loss method score, which indicates the degree of weight-loss method use, was associated with PPD. These results indicate using weight-loss methods before pregnancy is associated with an increased risk of PPD in Japanese women.

www.nature.com/scientificreports/ Some women experience depressive symptoms after pregnancy, and some women develop PPD. Recently, the estimated prevalence rate of PPD is 15.1% among pregnant Japanese women at 1 month after delivery 11 and 17.7% worldwide 12 . PPD begins within 4 weeks after delivery, with symptoms including sadness, anxiety, irritability, loss of interest, anorexia, appetite disturbance, excessive or lack of concern for the baby, and constant fatigue 13 . These symptoms adversely affect mother-infant interaction, breastfeeding, sleep routines, infant health care, and safety practices 14 . Additionally, children who have mothers with PPD exhibit an increased risk of behavioral problems in childhood, depression, and a decline in mathematics performance during adolescence 15 . Therefore, it is important to detect and treat PPD among postpartum women in the early stages.
To prevent PPD using a public health approach, risk factors of PPD should be investigated in detail. According to meta-analyses and systematic reviews [16][17][18][19] , risk factors of PPD include marital status, poor conjugal relations, a history of depression, psychological disturbance during pregnancy, stressful life events, unplanned pregnancy, low levels of social support, socioeconomic status, low self-esteem, neuroticism, and obstetric factors. Added to these factors, smoking 20 and intimate partner violence 21 have also been reported as risk factors. Although the risk factors of PPD have been well investigated, no studies have examined the association between weightloss behaviors during the year before the current pregnancy and PPD. Considering the association between depression and unhealthy weight-loss behavior, we hypothesized that unhealthy weight-loss behaviors are risk factors of PPD. Furthermore, because most women tend to try multiple weight-loss methods to lose weight, we hypothesized that women who engaged in multiple weight-loss methods may have a higher risk of PPD. To investigate these hypotheses, we analyzed data from the Japan Environment and Children's Study (JECS), a nationwide birth cohort study in Japan.

Methods
Study design and participants. The aim of the JECS, an ongoing prospective birth cohort study that began in 2011, is to evaluate the effects of various environmental factors on the health and development of children. Kawamoto et al. (2014) reported the JECS protocol and its details 22 . In brief, the JECS recruited 97,413 pregnant women living in the study areas (15 regional centers, 19 prefectures) between January 2011 and March 2014. The JECS protocol was reviewed and approved by the Ministry of the Environment's Institutional Review Board on Epidemiological Studies and the Ethics Committees of all participating institutions (No. 100910001). The JECS is conducted in accordance with the Declaration of Helsinki and Ethical Guidelines for Medical and Biological Research Involving Human Subjects in Japan. Written informed consent was obtained from all participants involved in the study.
In this study, we used a JECS dataset (jecs-ta-20190930) released in October 2019. The dataset does not contain any identifying patient information. In total, 104,062 fetal records were registered in 19 prefectures of Japan (Aichi, Chiba, Fukushima, Fukuoka, Hokkaido, Hyogo, Kanagawa, Kochi, Kumamoto, Kyoto, Miyagi, Miyazaki, Nagano, Okinawa, Osaka, Shiga, Toyama, Tottori, and Yamanashi) between January 2011 and March 2014. We excluded 3758 women because of miscarriage, stillbirth, or missing data on pregnancy, 1891 women with multiple birth, 5468 women who participated in the JECS multiple times, and 14,048 women because of a medical history of psychological disorders (depression, dysautonomia, anxiety disorder, schizophrenia, epilepsy, migraine, and other disorders) or developmental disorders (attention-deficit/hyperactivity disorder, learning disability, autism, and other disorders). Moreover, we excluded 16,451 women who did not complete the questionnaire. After exclusion, we analyzed complete data from 62,446 women (Fig. 1). We issued a questionnaire to each participant during their first trimester of pregnancy, either in person when the participant was recruited or by post, after explaining the purpose and practicalities of the study. Follow-up questionnaires were issued in person or by post during the second or third trimester, shortly after childbirth, and at 1 month postpartum during scheduled in-patient hospital checkups.

Assessment of PPD.
We used the Japanese version of the Edinburgh Postnatal Depression Scale (EPDS) to assess PPD. The EPDS is used worldwide for screening PPD. Data were collected from participants 1 month after delivery. The EPDS comprises 10 items, and responses are given to each item using a four-point scale (from 0 to 3). Higher scores indicate more severe depressive symptoms 23 . An optimal cut-off score of 8/9 has been reported for Japanese women at 1 month after delivery (75% sensitivity and 93% specificity) 24 . Therefore, we defined PPD at 1 month after delivery as an EPDS score ≥ 9.

Assessment of weight-loss behavior.
To assess weight-loss behavior during the year before the current pregnancy, a dichotomous questionnaire was administered to participants during their second or third trimester of pregnancy. Participants were asked: Did you engage in any of the following weight-loss behaviors during the year before current pregnancy? Multiple responses were possible. We defined women as using a weight-loss method if they checked at least one of the following weight-loss methods: eating two-thirds as much as usual or less, avoiding snacking and midnight snacking, eating specific diet foods, taking a diet pill, vomiting after eating, smoking cigarettes, and engaging in exercise. Participants answered yes or no for each item.
Calculation of weight-loss score. To investigate the association between multiple weight-loss behaviors and PPD, we defined a weighted weight-loss method score. In a previous paper, weight-loss behaviors were classified as healthy and unhealthy weight-loss behavior 10 . Following that paper, we defined three types of weightloss methods: we defined engaging in exercise and avoiding snacking and midnight snacking as weight-loss methods that were healthy; eating specific diet foods and eating two-thirds as much as usual or less as unhealthy weight-loss methods; taking a diet pill, vomiting after eating, and smoking cigarettes as extremely unhealthy weight-loss methods. In determining weight-loss method scores, we focused on the number of weight-loss www.nature.com/scientificreports/ method used and unhealthy weight-loss behaviors. To assess an excess degree of weight-loss method use, the weight-loss method score was calculated as the total value, with 1 point for healthy weight-loss methods, 2 points for unhealthy weight loss methods, and 3 points for extremely unhealthy weight-loss methods. The total score indicated the degree of weight-loss method use.
Other confounding factors. Confounding factors comprised the following variables, obtained from the medical records: pre-pregnancy BMI (≤ 18.5, 18.5-24.9, ≥ 25 kg/m 2 ), age of the mother (≤ 19, 20-24, 25-29, 30-34, 35-39, or ≥ 40 years), child's sex (male or female), mode of delivery (spontaneous delivery, induced delivery, vacuum extraction/forceps delivery, or planned cesarean delivery/emergency cesarean delivery), number of previous deliveries (nullipara or multipara), diseases in the child currently under treatment (yes or no), how the mother became pregnant this time (spontaneously, ovulation induction through medication, artificial insemination in vitro fertilization, intracytoplasmic sperm injection, fresh embryo transfer, frozen embryo transfer, blastocyst transfer), recurrent miscarriage (yes or no), and gestational weight gain (normal weight gain or abnormal weight gain; abnormal weight gain was defined following the Optimal Weight Gain Chart for Pregnancy published by the Ministry of Health, Labour and Welfare in 2006 25 ). Covariates comprised the following variables, obtained using self-administered questionnaires completed by participants: current marital status (married, single, divorced/widowed), feelings when learning about the pregnancy (very happy, surprised but happy, surprised and confused, upset, did not have any specific or other feelings), experience of any stressful events during the past year (yes or no), having someone who can be counted on to provide emotional support (never, rarely, some of the time, most of the time, or all of the time), alcohol intake (never, past, or current), highest level of education (junior high school/high school, technical junior college/technical or vocational college/associate degree, or bachelor's degree/graduate degree), annual household income (< 4 million JPY, 4 to < 6 million JPY, or ≥ 6 million JPY), receiving emotional abuse from husband/romantic partner during current pregnancy (yes or no), receiving physical abuse from husband/romantic partner leading to injury during current pregnancy (yes or no). Women's antenatal psychological distress was assessed using the Japanese version of the Kessler 6 scale (K6) 26 . The data were collected during the first trimester. The K6 comprises six items, with higher scores indicating more severe psychological distress. An optimal cut-off score of 12/13 has been reported 26,27 ; therefore, we defined antenatal psychological distress as a K6 score ≥ 13.
Statistical analysis. The crude odds ratio and adjusted odds ratio (aOR) were calculated in binomial logistic regression analyses. Briefly, to calculate the crude odds ratio, a model was constructed using PPD as the dependent variable and the weight-loss method or weight-loss method score as independent variables. To cal- www.nature.com/scientificreports/ culate the aOR, a model was constructed using PPD as the dependent variable and the weight-loss method or score and confounding factors that were significantly associated with PPD (according to the Mann-Whitney U test or chi-square test) as independent variables. Significance was defined as p < 0.05, and 95% confidence intervals (CIs) were calculated. As a sensitivity analysis to address the influence of missing values, we used a multiple imputation method. Briefly, 100 simulated datasets were generated in a multiple imputation method using the R mice package 28 . Each simulated dataset was analyzed in logistic regression analysis; each estimation was combined with pooling rules 29 . To assess the presence of collinearity, we calculated the variance inflation factor (VIF) and checked for values < 10. The VIF values were as follows: age of the mother:  Table 1 and Supplementary Table S1 show the main characteristics of the 62,060 participants according to EPDS scores, and the complete characteristics of 78,897 participants with missing values, respectively. At 1 month after delivery, 7464 (12%) women had PPD (EPDS ≥ 9) and 54,596 (88%) women did not have PPD.

Results
To elucidate the association between using at least one weight-loss method and PPD, we performed logistic regression analysis (Table 2). We defined the reference group as women who did not engage in any weight-loss behaviors. Because PPD is associated with depression during pregnancy, we stratified psychological distress during the second or third trimester. In women who did not have psychological distress during the second or third trimester (K6 < 13), the aOR for PPD was 1.318 (95% CI: 1.246-1.394) in those using at least one weight-loss method. In women with psychological distress during the second or third trimester (K6 ≥ 13), the aOR for PPD was 1.250 (CI: 0.999-1.565) in those using at least one weight-loss method. Although there was no significant association between PPD and using at least one weight-loss method in women with psychological distress during pregnancy, there was a positive trend between PPD and weight-loss behaviors before pregnancy because of the 95% CI was 0.999 to 1.565.
Next, there is the possibility that the impact on PPD was varied depending on the type of weight-loss behavior before pregnancy. To elucidate this, a logistic regression analysis was performed between PPD and each weightloss method. In univariate logistic regression analysis, there was significant association between each weightloss methods and PPD (Table 3). Even if we performed multivariate logistic regression analysis using potential confounding factors, we could find the association between each weight-loss methods and PPD (Table 3). These results indicated that all weight-loss methods before pregnancy were significantly associated with PPD.
Although we adjusted for BMI before pregnancy in logistic regression analysis, the association between PPD and use of weight-loss method may differ by pre-pregnancy BMI. To clarify this, we performed logistic regression analysis after stratification by pre-pregnancy BMI (Fig. 2, Supplementary Table S2). We separated out three datasets from the original dataset according to BMI before pregnancy (underweight: BMI < 18.5 kg/m 2 ; normal weight: BMI 18.5 to < 25 kg/m 2 ; overweight or obese: BMI ≥ 25 kg/m 2 ). Interestingly, we found a significant association in underweight women between PPD and eating two-thirds as much as usual or less, avoiding snacking and midnight snacking, eating specific diet foods, and smoking cigarettes (Fig. 2a, Supplementary Table S2). PPD was not associated with vomiting after eating or engaging in exercise among underweight women. In women with normal BMI, all weight-loss methods were associated with PPD (Fig. 2b, Supplementary Table S2). In overweight or obese women, we found a significant association between PPD and smoking cigarettes only; there was no association between PPD and other methods (Fig. 2c, Supplementary Table S2). These results indicated that the association between weight-loss methods and PPD varied according to BMI before pregnancy.
To elucidate the association between using multiple weight-loss methods and PPD, we calculated the weightloss method score. The weight-loss method score indicates the weighted degree of weight-loss method use and a weight-loss score of 0 indicates no weight-loss method used. To investigate the effect of weight-loss score on PPD, we included a dummy variable for weight-loss score and performed logistic regression analysis. Although we could observed the significant association between weight-loss score and PPD compared with the women with a weight-loss score of 0, the aORs were fluctuated in all BMI group (Fig. 3, Supplementary Table S3). In underweight women, the aOR was increased, with a score of 3. A weight-loss score of 3 indicated use of healthy weight-loss method and one unhealthy weight-loss method. Therefore, using at least one unhealthy weight-loss method was associated with PPD at 1 month after delivery. In normal-weight women who engaged in multiple weight-loss behaviors, weight-loss score was significantly associated with an increased risk of PPD at 1 month after delivery. From score 1 point to 5 points, the ORs increased in a score-dependent manner. In overweight/ obese women, the weight-loss score of 4, which indicated use of multiple healthy weight-loss method and at least one unhealthy weight-loss methods or two unhealthy weight-loss methods, was significantly associated with PPD. These results showed that excessive use of multiple weight-loss methods and unhealthy weight-loss methods before pregnancy were associated with PPD at 1 month after delivery. www.nature.com/scientificreports/ We included only participants with a complete dataset and excluded those with missing data (Fig. 1). However, there was a possible effect of missing values on our results. To clarify this, we performed logistic regression analysis using a simulated dataset, with missing values generated using multiple imputation methods (Supplementary  Tables S4 and S5, Supplementary Figs. S1 and S2). The results were the same using the simulated dataset; thus, missing values did not affect the results in this study.

Discussion
There are no studies on the association between PPD and weight-loss behaviors before pregnancy in Japanese women. We performed logistic regression analysis using PPD and weight-loss behaviors and using JECS data. The results revealed a significant association between weight-loss behaviors before pregnancy and PPD. The Table 2. Odds ratio (95% CI) of PPD according to use of weight-loss methods, after excluding participants with a medical history of psychiatric disorders. Cl confidence interval, aOR adjusted odds ratio, PPD postpartum depression, K6 Kessler 6-Item Psychological Distress Scale, BMI body mass index. a Adjusted for age of the mother, sex, mode of delivery, number of previous deliveries, marital status, feelings when learning about pregnancy, stressful events, emotional support, highest level of education, annual household income, emotional abuse from partner, physical abuse from partner, BMI in first trimester, disease in the child currently under treatment, how the mother became pregnant this time, recurrent miscarriage, and gestational weight gain.  www.nature.com/scientificreports/ association between using weight-loss methods and PPD varied according to BMI before pregnancy. Using multiple weight-loss methods were significantly associated with the increasing the risk of PPD. These results may indicated that weight-loss behaviors increased the PPD risk for Japanese women. It is important to educate women considering pregnancy about the dangers of excessive or unhealthy weight-loss behaviors.
In general, it is reported that women should begin a pregnancy with a healthy body weight because of the increased risk of pregnancy complications 31 . In overweight or obese women, it is recommended to reach a healthy weight before conceiving, such as with counseling on diet and physical activity 31 . However, our results indicated a significant association between weight-loss behaviors before pregnancy and PPD (Table 2). This result seemed to contradict the statement about weight-loss behavior before pregnancy to reach a healthy weight. However, our results also indicated the association between PPD and weight-loss behavior was varied in different BMI groups before pregnancy (Fig. 2, Supplementary Table S2). Interestingly, there was no association between weight-loss behavior and PPD in overweight/obese women (Fig. 2c, Supplementary Table S2). We noted that only smoking . Adjusted odds ratio and 95% confidence interval was calculated by multivariate logistic regression analysis. Error bar represents 95% confidence interval. Dotted line indicates the reference category (women who did not engage in any weight-loss behaviors). Adjusted for age of the mother, child's sex, mode of delivery, number of previous deliveries, marital status, feelings when learning about the pregnancy, stressful events, emotional support, highest level of education, annual household income, emotional abuse from partner, physical abuse from partner, diseases in the child currently under treatment, how the mother became pregnant this time, recurrent miscarriage, psychological distress, and gestational weight gain. Fully information such as adjusted odds ratio, 95% confidence interval and number of women in each groups were indicated supplemental Table 2. PPD postpartum depression, BMI body mass index. www.nature.com/scientificreports/ cigarettes was associated with PPD ( Fig. 2c, Supplementary Table S2). Thus, our results underscore the importance of weight-loss behavior before pregnancy. In normal-weight women, unhealthy weight-loss behaviors such as taking a diet pill, smoking cigarettes, and vomiting after eating were associated with PPD, and there was an association between smoking cigarettes and PPD in underweight women. Because smoking cigarettes is associated with PPD but not the goal of weight loss, this result was consistent with those of previous studies 20 . Other extremely unhealthy weight-loss behaviors, such as taking diet pills and vomiting after eating, were associated with PPD in normal-weight women. Although the precise mechanism is unknown, these results indicated that eating behaviors related to weight loss before pregnancy may be associated with PPD, at least in normal-weight women. Therefore, to prevent PPD, it is important to raise awareness among women about the risk of using weight-loss methods according to their pre-pregnancy BMI.
It was previously reported that unhealthy weight-loss behaviors were associated with psychological disorders 9,10 . Eating disorders, such as binge eating disorder and bulimia nervosa, are associated with PPD 32 . Although vomiting itself may not be related to losing weight or to eating disorders, some studies have found a Figure 3. Risk of postpartum depression was increased with increased weight-loss method score in a dependent manner. (a) Association between weight-loss method score before pregnancy and PPD in underweight women (N = 9726). (b) Association between weight-loss method score before pregnancy and PPD in normal-weight women (N = 45,945) (c) Association between weight-loss method score before pregnancy and PPD in overweight/obesity women (N = 6389). Adjusted odds ratio and 95% confidence interval was calculated by multivariate logistic regression analysis. Error bar represents 95% confidence interval. Dotted line indicates the reference category (women who did not engage in any weight-loss behaviors). Adjusted for age of the mother, child's sex, mode of delivery, number of previous deliveries, marital status, feelings when learning about the pregnancy, stressful events, emotional support, highest level of education, annual household income, emotional abuse from partner, physical abuse from partner, diseases in the child currently under treatment, how the mother became pregnant this time, recurrent miscarriage, psychological distress, and gestational weight gain. Fully information such as adjusted odds ratio, 95% confidence interval and number of women in each groups were indicated supplemental Table 3 www.nature.com/scientificreports/ positive association between vomiting owing to nausea during pregnancy and PPD 33,34 . However, there are no studies on a direct association between vomiting to lose weight before pregnancy and PPD. In this study, vomiting after eating was associated with PPD in women with normal BMI but not in underweight and overweight/ obese women (Table 3, Fig. 2, Supplementary Table S2). Because eating disorders occur intercurrently with other psychological disorders, we excluded participants with a medical history of psychological disorders and developmental disorders from the data analysis; this group may have included women with a history of eating disorders. Thus, our results indicated that extreme weight-loss behaviors, but not those owing to eating disorders, may be directly associated with PPD in normal-weight women. Generally, exercise is a protective factor for mental health. A previous study reported that physical activity during pregnancy reduced the risk of PPD 35 . Surprisingly, however, weight-loss behaviors that were not unhealthy, such as avoiding snacking and midnight snacking and engaging in exercise, were also associated with PPD in normal-weight women (Fig. 2b, Supplementary Table S2). These results are inconsistent with those of previous studies. We queried participants regarding whether they engaged in exercise as a weight-loss method. Because approximately 90% of Japanese women in their 20s and 30s are underweight or normal weight, most Japanese women are either thin or within the normal weight range 1 . In this study, 55,671 (89.7%) pregnant women were not obese before pregnancy (Table 1). Interestingly, exercise was not associated with PPD in overweight/obese women (Fig. 2c, Supplementary Table S2). This indicates that despite most women in Japan not needing to lose weight, many engage in weight-loss behaviors, such as excessive exercise and excessive limitation of nutrient intake, and these excessive behaviors may be associated with PPD. Additionally, the desire to be thin is common among many women. The desire to lose weight may be associated with the risk of PPD, but further studies are needed to investigate this relationship.
The current findings revealed an association between weighted weight-loss method scores and the risk of PPD in women with any BMI (Fig. 3, Supplementary Table S3). This was the first study to report that the number of weight-loss methods used affects PPD, with a higher PPD risk associated with an increasing number of methods used in normal-weight women. Women with weight-loss scores of more than 3 points engaged in multiple weight-loss behaviors, such as trying both healthy and unhealthy ways to lose weight. In normal-weight women, engaging in healthy as well as unhealthy weight-loss behavior associated with increase the risk of PPD, as compared with engaging in one weight-loss behavior (Fig. 3b, Supplementary Table S3). Because women who undertake weight loss using multiple methods often engage in extreme weight-loss behaviors, they may experience physical and psychological problems, such as poor nutrition and eating disorders. Additionally, women who use multiple weight-loss methods may be obsessed with their body image and feel the need to lose weight. Such physical and psychological problems before pregnancy may lead to PPD after delivery in women with any BMI. Interestingly, the associations between weight-loss methods and PPD were varied by pre-pregnancy BMI (Figs. 2,  3, Supplementary Tables S2, S3). In overweight/obese women, weight-loss behavior except smoking cigarettes was not associated with PPD (Fig. 2c, Supplementary Table S2). These results may indicate that the desire to be thin in normal and skinny women is stronger than that in overweight/obese women. The desire to be thin may be associated with PPD in women with normal and low BMI values. Therefore, awareness among women about the risk of using extreme weight-loss methods is important to prevent PPD and psychological problems.
There are six limitations in this study. First, weight-loss behavior was assessed using dichotomous-variable questions. Therefore, we could not obtain information regarding the frequency, degree, and time frame of weightloss behaviors using the questionnaires. Nevertheless, these questions have been used in several studies 10,36,37 . Therefore, we believe that the use of dichotomous-variable questions did not pose a problem in this study. To reveal further details regarding weight-loss behaviors before pregnancy, further studies are warranted. Second, we collected information about weight-loss behavior before pregnancy when participants were in their second or third trimester of pregnancy, which could introduce recall bias. For example, women who were not overly concerned about their body image may report engaging in no weight-loss behaviors even if weight-loss methods were used. To remove any bias, objective indicators of weight-loss methods should be used to clarify this. Third, we could not obtain BMI data at the time that our participants began to use weight-loss methods. Therefore, we could not include the success or failure of a weight-loss method as adjustment variables. However, we observed a significant association between weight-loss behavior and PPD, even with stratification of BMI before pregnancy. Because approximately 90% of Japanese women in their 20s and 30s are within the normal weight range or below, there may be an association between PPD and extreme or excessive weight-loss behavior. Further studies are required to clarify the associations among PPD, weight-loss behavior, and BMI in detail. Fourth, we could not completely eliminate the effect of eating disorders in the analysis. We excluded participants with psychological disorders and developmental disorders from the data analysis; however, we may not have eliminated participants with borderline eating disorders from the analysis. To clarify the precise association between eating disorders and PPD, further investigation is needed using questionnaires on eating disorders, such as the Eating Attitudes Test-26. Fifth, we could not measure the desire to be thin among women in this study. This desire may be associated with PPD. To clarify the precise association, further research is needed. Sixth, we used weighted weight-loss scores. There was a fluctuation in the aOR between PPD and weighted weight-loss scores, especially in underweight women (Fig. 3, Supplementary Table S3). This is an important limitation of this score. Because we calculated the total score in the analysis, unhealthy weight-loss behavior and multiple healthy weight-loss methods (e.g., a score of 3 included women who used three healthy weight-loss methods and those who used one extremely unhealthy weight-loss method) were treated equally. However, it is unknown if these are actually equivalent. Thus, there might be a fluctuation in the aOR between PPD and weighted weight-loss scores. To elucidate this, new precisely validated weight-loss scores should be developed, and further studies are needed.
Despite these limitations, the strengths of the current study included the large sample size in Japan and prospective data collection. To the best of our knowledge, this study was the first to investigate the association between weight-loss behaviors in the year before pregnancy and the risk of PPD in a large sample of women www.nature.com/scientificreports/ throughout Japan. Our findings provide further evidence regarding the health risks of unhealthy weight-loss behavior among Japanese women.
In conclusion, the current study revealed that using weight-loss methods in the year before pregnancy was associated with an increased risk of PPD in Japanese women. Many Japanese women have a desire to lose weight and may have difficulty avoiding weight-loss behaviors. The media have created an image in society that women must lose weight and be thin. However, our findings highlight the risks associated with unhealthy and excessive weight-loss behavior among Japanese women who plan to become pregnant in the near future. It is important to educate Japanese women, particularly those planning pregnancy, regarding the association between weight-loss behaviors and mental health.

Data availability
The data in this study are unsuitable for public dissemination owing to ethical restrictions and the legal framework in Japan. This is prohibited by the Act on the Protection of Personal Information (Act No. 57 of 30 May 2003, amended on 9 September 2015) to publicly deposit data containing personal information. The Ethical Guidelines for Medical and Health Research Involving Human Subjects, enforced by the Japan Ministry of Education, Culture, Sports, Science and Technology and the Ministry of Health, Labour and Welfare, also restrict open sharing of the epidemiologic data. All inquiries about access to the data should be sent to: jecs-en@nies. go.jp. The person responsible for handling enquiries is Dr Shoji F. Nakayama, JECS Programme Office, National Institute for Environmental Studies.